JC-1 Mitochondrial Membrane Potential Assay

JC-1 Mitochondrial Membrane Potential Assay - Cell Detection Reagent JC-1 Assay detects the mitochondrial membrane potential changes in cells by the cationic, lipophilic JC-1 dye. In healthy cells, JC-1 enters the energized mitochondria and form aggregates which change the fluorescent property of JC-1 dye. JC-1 monomers exhibit green fluorescence whereas the JC-1 dye aggregates also called J-aggregates show intense red fluorescence. Unhealthy or apoptotic cells have low mitochondrial membrane potential. JC-1 does not form aggregates in mitochondria’s with low membrane potential and remain in monomeric form and exhibit green fluorescence. Thus higher the ratio of red to green fluorescence, the higher is the polarization of mitochondrial membrane. Size 100 Assays Product Category Cell Detection Kit Components 1. JC-1 in DMSO 2. Staining Buffer 3. CCCP (100mM) 4. Ultrapure Water Storage/Stability 2 ~ 8°C/1 year Shipping Gel Packs Intend use : • 12% GST extra on Product • Packaging, Handling & Transport charges (with GST) extra as applicable. • Delivery period : 3-4 weeks or earlier as per availability.